By Prof. Dr. K. A. Jellinger (auth.), Prof. Dr. Kurt A. Jellinger, Dr. Manfred Windisch (eds.)
This quantity provides an summary of the current country of paintings at the type, neuropathology, scientific presentation, neuropsychology, analysis, neuroimaging and healing percentages in non-Alzheimer’s dementias, an more and more very important crew of CNS ailments, which account for 7 to 30% of dementing problems in adults and elderly matters, and therefore, symbolize the second one so much widespread reason behind dementia after Alzheimer’s ailment. The monograph presents the most recent info for neurologists, psychiatrists, dementia study employees, dementia clinicians, neuropathologists, neurobiologists, and practising physicians.
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Extra resources for New Trends in the Diagnosis and Therapy of Non-Alzheimer’s Dementia
For all other antibodies, staining was best on thicker vibratome sections. 5 cm blocks of tissue were immersion fixed in 4 % paraformaldehyde for 12 to 24 hours or in Bouin's solution for 1-2 hours. 1 % azide before sectioning. For staining of floating sections, peroxidase-, ~-galactosidase- or alkaline phosphatase-conjugated secondary antibodies were used. The secondary antibody was specific to the species and the isotype of the primary antibody. Sections were usually incubated for 16 hours at 4°C.
Eur Arch Psychiat Clin Neurosci 245: 299305 Schneider JA, Gearing M, Watts R, Mirra SS (1995) Corticobasal degeneration: a neuropathological, clinical and molecular study (Abstract). J Neuropathol Exp Neuro154: 446 Schneider JS, Pope-Coleman A (1995) Cognitive deficits precede motor deficits in a slowly progressing model of parkinsonism in the monkey. Neurodegeneration 4: 245255 Scoones D, Ince PG, Shaw PJ, Perry RH (1995) Neuritic pathology in Huntington's disease is present in the substantia nigra in addition to the cortex: observations in 21 cases, including the rigid variant (Abstract).
For some antibodies assessment of adequacy of staining was possible due to the presence of tissue "internal controls". For example, ubiquitin-positive granular degeneration of myelin is an invariant age-related change. If the white matter lacked this type of staining, the stain quality was considered inadequate and was repeated. For neurofilament antibodies, a similar internal control was staining ofaxons in white matter. For antibodies that 34 D. W. Dickson et al. Table 1. Antibodies used for immunocytochemistry Antibody SMI- 31 NP18 Alz-50 PHF-1 Ab39 TG-3 UBQ Isotype & Dilution Specificity neurofilament (phosphorylated epitope) neurofilament (phosphorylated epitope) tau (non phosphorylated epitope) tau (phosphorylated epitope) NFT (conformational epitope) PHF (phosphorylated epitope) ubiquitin (polyc1onal antibody) mIgG1 1:5,000 mIg]M 1:10 mIgM 1:10 mIgG1 1:200 mIgG1 1:10 mIgM 1:10 rIg 1:400 Source Sternberger-Meyer, Inc.