Membrane Fusion in Fertilization, Cellular Transport, and by Felix Bronner (Eds.)

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By Felix Bronner (Eds.)

Meant for mobile, molecular and developmental biologists, biochemists, physiologists, neuroscientists, and microbiologists, this quantity discusses such themes because the function of GTP-binding proteins in sign transduction, and calcium pumps within the plasma and intracellular membranes,

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Ferfil. Stevil. 3, 13-26. Boatman, D. E.. and Bavister, B. D. (1984). Stimulation of rhesus monkey sperm capacitation by cyclic nucleotide mediators. J . Reprod. Fertil. 71, 357-366. Boldt, J . and Wolf, D. P. (1986). An improved method for isolation of fertile zona-free mouse eggs. Comere Rrs. 13, 213-222. Carrol. E. J.. and Levitan, H. (l978a). vloc~r~tirrorrrs piirpurlitus, is inhibited by fluorescein dyes. Deu. Biol. 63, 432-440. Carrol, E. J . and Levitan. H . (1978b). Fertilization is inhibited in five diverse animal phyla by erythrosin B.

B i d . Rrprod. 6, 300-309. Hinkley. R . E.. Wright, B. D.. and Lynn. J. W. (1986). Rapid visual detection of sperm-egg fusion using the DNA-specific fluorochrome Hoechst 33342. Dru. B i d . 118, 148-154. , and Yanagimachi, R . (1978a). Temperature dependence of sperm-egg fusion and post-fusion events in hamster fertilization. J . Exp. Zoo/. 205, 433-438. , and Yanagimachi, R . (1978b). Effects of various enzymes on the ability of hamster egg plasma membrane to fuse with spermatozoa. Gmnere Rrs.

5-1 mM), Yanagimachi (198 I ) has been unable to confirm this in the hamster-. F. Cytochalasin According to Longo (1978), sea urchin eggs treated with cytochalasin B at concentrations of 1-10 pg/ml (2-20 p M ) are activated by spermatozoa but fail to incorporate sperm heads into the cytoplasm. It is reasonable to assume that sperm-egg fusion has taken place, but, because the cytoskeletal (actin) system has become disorganized, the fertilization cone has failed to form, so that the sperm head is not taken up (Vacquier, 197%).

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