Leukaemia Diagnosis 4th ed. by Barbara J. Bain

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By Barbara J. Bain

Up to date sensible advisor TO LEUKAEMIA DIAGNOSISWritten by means of a well known specialist this sensible consultant were totally revised and up to date. The e-book covers fresh advances within the fields of immunophenotyping, cytogenetics and molecular genetics. It illustrates how laboratory innovations are used for the prognosis and category of leukaemia and comprises pictures of irregular cells to help diagnosis.This fourth edition:Incorporates the options of the 2008 WHO category of Tumours of Haematopoietic and Lymphoid TissuesIncludes three hundred top of the range complete color electronic photographs of irregular cells in leukaemia and lymphoma -   50 of that are thoroughly newEvery haematologist and haematopathologist should still make a copy shut handy for fast reference.

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Fig. 58 BM film from a patient with FAB L1 ALL. MGG ×100. CYTOLOGY, CYTOCHEMISTRY AND THE FAB CLASSIFICATION 51 Fig. 59 Ultrastructure of lymphoblasts in FAB L1 ALL. ) numbers of azurophilic, but peroxidase-negative, granules. 62. About a quarter of cases of ALL fall into the L2 category. L2 ALL may be of B or T lineage. Fig. 60 BM film from a patient with FAB L2 ALL showing large pleomorphic blasts; the cells were CD10 positive. MGG ×100. abundant. The nuclei are irregular in shape with clefting, folding and indentation being common, and with heterogeneity also of the chromatin pattern.

47. With the exception of cases among children with Down syndrome, the prognosis in both children and adults appears to be poor [30,67,68]. Cytochemical reactions in M7 acute myeloid leukaemia Megakaryoblasts are negative for MPO, SBB and CAE. The more mature cells of this lineage are PAS positive and have partially fluoride-sensitive NSE activity, demonstrated with ANAE. ANBE activity is demonstrable in only a minority of cases [67]. On PAS staining there are positive granules on a diffusely positive background.

A minimum of 5% of bone marrow eosinophils has been suggested as a criterion for the recognition of significant 42 CHAPTER 1 Fig. 44 Ultrastructural cytochemistry of a blast cell showing a positive platelet peroxidase reaction. ) (a) (b) Fig. 45 PB and BM films from a patient with FAB M7 AML presenting as acute myelofibrosis; the nature of the leukaemia was demonstrated by a positive reaction for platelet peroxidase. (a) PB film showing mild anisocytosis and a blast cell with no distinguishing features.

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