By Joseph Feder
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22: 362-365. , and Gilbride, K. (1984). Manuscript in preparation. Glacken, M. , Fleischaker, R. , and Sinskey, A. (1983). Mammalian Cell Culture: Engineering Principles and Scale-Up, Trends in Biotechnology, 1: 102-108. , (1984). Novel Bioreactor Systems and their Applications, Biotechnology 2: 447-453. Lim, F. , (1980). Microencapsulated Islets as Bioarticiial endocrine Pancreas, Science, 210: 908-910. Jarvis, A. and Grdina, T. (1983). Production of Biologicals from Microencapsulated Living Cells, Biotechniques 1, 22-27.
Effect of initial antibody purity on subsequent purification. Intracapsular materials with different antibody purities were concentrated separately by precipitation with 60% ammonium sulfate and collected by centrifugation at 15000 X g for 15 minutes. The ammonium sulfate pellets were dissolved and dialyzed exhaustively in a Tris/NaCl buffer. Samples of each were passed over DE-53 cellulose - ion exchange columns. The unbound material, containing the antibody, was collected and assayed for total protein and antibody concentration.
Thousand Oaks, CA): Have you looked at the viability of the cells inside the beads with time? It appears that you had a constant rate of production of antibody even though the cell density was increasing, suggesting that the specific productivity was decreasing. Do you think that is a viability related phenomena? DR. R. G. RUPP: The actual calculation, I think, was done correctly. It was based on viable cells. But to answer your question, honestly, the cell viability is decreasing from about day 12.