Immunoenzyme Multiple Staining Methods by C. M. Van Der Loos

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By C. M. Van Der Loos

This booklet describes double and triple immunoenzyme staining equipment which might be played with commercially on hand reagents, and offers primary protocols which are simply tailored to many various purposes. It offers recommendation for which stains to choose, how they are often utilized, and priceless trouble-shooting recommendation to steer a brand new consumer within the box. "Immunoenzyme a number of Staining tools is a straightforward and crucial consultant in the course of the many alternative a number of staining tools and color mixtures to be had, that can frequently be seen as advanced and complicated. it's a useful useful better half for mobile biologists, pathologists and histologists new to a number of staining, and is usually an invaluable reference for more matured staff.

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Verrucosum- the most common animal dermatophyte in Czechoslovakia. XI. CULTIVATION A prerequisite for establishing a diagnosis of veterinary dermatophytoses is the demonstration by cultivation of the causative agent. g. ). In this way a differential diagnosis may be made. The limited reliability of the results of microscopic examination of samples taken from cutaneous lesions of animals (see above) emphasizes that the cultivation must be performed consistently. ). ). For species identification it is necessary to isolate the dermatophyte from the lesion, to obtain a colony.

Its exposure to the iodine solution. In-this time the changes in the structure of the macroconidia caused by iodine are completed. remain intact, ·but their structure cannot be satisfactorily distinguished. The cellular wall of tlie macroconidia · seems to thicken after a 10 min exposure to iodine, but these changes seem to be constant and, therefore, the use of iodine solution may be permitted although even artificial ·blues ma,y be obtained. Table 13 shows the number ofmacroconidia produced by the individual dermatophytes.

No differences exist between T. mentagrophytes and T . m . var. interdigitale and between T. terrestre and T . verrucosum. As evident from the Table, hairs are frequently d'sintegrated by both coaxial and rectangular keratinolysis or they may remain intact. The method must be considered as supplementary and its diagnostic value must be verified. 4. Nutritional tests AJELLO et al. (1963) summarized the nutritional patterns of some Trichophyton species. It has been shown that it may be possible to use these tests for identification (Table 19).

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