Gene Cloning and DNA Analysis: An Introduction (6th Edition) by T. A. Brown

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By T. A. Brown

Identified world-wide because the regular introductory textual content to this significant and fascinating region, the 6th version of Gene Cloning and DNA research addresses new and turning out to be components of study while keeping the philosophy of the former versions. Assuming the reader has little previous wisdom of the topic, its significance, the rules of the ideas used and their purposes are all rigorously laid out, with over 250 in actual fact provided four-colour illustrations.
In addition to a couple of informative adjustments to the textual content during the booklet, the ultimate 4 chapters were considerably up to date and prolonged to mirror the notable advances made in recent times within the purposes of gene cloning and DNA research in biotechnology.

Gene Cloning and DNA research is still an important introductory textual content to a variety of organic sciences scholars; together with genetics and genomics, molecular biology, biochemistry, immunology and utilized biology. it's also an ideal introductory textual content for any expert desiring to benefit the fundamentals of the topic. All libraries in universities the place clinical, lifestyles and organic sciences are studied and taught must have copies to be had on their cabinets.

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Extra resources for Gene Cloning and DNA Analysis: An Introduction (6th Edition)

Sample text

As can be imagined, skill and experience are needed to judge the matter to perfection. 18). The problem now is to reduce the size of the suspension to 5 ml or less, a manageable size for DNA extraction. Phage particles are so small that they are pelleted only by very high speed centrifugation. Collection of phages is therefore usually achieved by precipitation with polyethylene glycol (PEG). This is a long-chain polymeric compound which, in the presence of salt, absorbs water, thereby causing macromolecular assemblies such as phage particles to precipitate.

Coli, new phage particles are continuously assembled and released from the cell. The M13 DNA is not integrated into the bacterial genome and does not become quiescent. With these phages, cell lysis never occurs, and the infected bacterium can continue to grow and divide, albeit at a slower rate than uninfected cells. 8 shows the M13 infection cycle. Although there are many different varieties of bacteriophage, only e and M13 have found a major role as cloning vectors. We will now consider the properties of these two phages in more detail.

21 Collection of phage particles by polyethylene glycol (PEG) precipitation. 20c). As can be imagined, skill and experience are needed to judge the matter to perfection. 18). The problem now is to reduce the size of the suspension to 5 ml or less, a manageable size for DNA extraction. Phage particles are so small that they are pelleted only by very high speed centrifugation. Collection of phages is therefore usually achieved by precipitation with polyethylene glycol (PEG). This is a long-chain polymeric compound which, in the presence of salt, absorbs water, thereby causing macromolecular assemblies such as phage particles to precipitate.

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