By Michael W. Stanley
Collage of Arkansas, Little Rock. textual content for medical pathologists on appearing superb needle aspiration (FNA). comprises suggestions, strategies, scientific ideas, sufferer interplay, and case stories. colour and halftone photomicrographs. DNLM: Biopsy, Needle - tools
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Additional info for Fine Needle Aspiration of Palpable Masses
The former involves alteration of the manufacturer's suggested staining times, to allow adequate fixation and staining of aspirated tissue fragments. 6. M o s t of the Romanowsky-stained smears illustrated in this book were prepared with this method. 6 Modified Diff-Quik® stain for rapid Romanowsky staining of aspiration smears: Applied to air-dried smears Reagent L Absolute methanol 1. I. Solution I 2. S. Solution II 3. \. Tap water 4. ). Slide examination 5. a. Coverslip with water (temporary) b.
It can then be collected and smeared, as described for tissue that was recovered from the needle hub. That is, small amounts can be handled with the two-step or modified two-step smearing techniques. If clotting occurs, a cell block should be prepared. An alternative to this simple method involves rinsing of the syringe and needle with saline or fixative. We rarely use this method, as the material obtained requires concentration by centrifugation or filtration, with its attendant disadvantages.
The top y far edge of the spreader slide is lowered onto the specimen droplet, and gentle pressure is used to flatten the specimen. 27. A typical smear produced by the one-step method is shown. This is best applied to smallvolume specimens. Small tissue particles are visible. 28. A typical specimen suitable for the two-step smear method: This drop of blood or other fluid containing cells or tissue particles is placed near the label end of a slide. 29. The end of the spreader slide is touched to the specimen slide at a 45° angle.