Difco manual on-line by Difco Laboratories

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Example text

1 Sodium Propionate . . . . . . . . . . . . . . . . 4 Dipotassium Phosphate . . . . . . . . . . . . . . 5 Magnesium Sulfate . . . . . . . . . . . . . . . . 1 Ferrous Sulfate . . . . . . . . . . . . . . . . 001 Bacto Agar . . . . . . . . . . . . . . . . . . . 2 at 25°C Glycerol Not applicable Precautions 1. For Laboratory Use. 2. MAY BE IRRITATING TO EYES, RESPIRATORY SYSTEM AND SKIN. (US) Avoid contact with skin and eyes.

Increasing amounts of the standard or the unknown and sufficient distilled or deionized water to give a total volume of 10 ml per tube, are added to the tubes containing 5 ml of the rehydrated medium. The appropriate volumes of the standards and their final concentrations are listed in the table. Measure the growth response turbidimetrically or titrimetrically. Turbidimetric readings are made after incubation at 35-37°C for 16-20 hours. Titrimetric readings are made after incubation at 35-37°C for 72 hours.

80 L-Cystine . . . . . . . . . . . . . . . . . . . . 1 L-Glutamic Acid . . . . . . . . . . . . . . . . . 6 L-Histidine Hydrochloride . . . . . . . . . . . . 124 DL-Phenylalanine . . . . . . . . . . . . . . . . 2 DL-Threonine . . . . . . . . . . . . . . . . . . 4 L-Tyrosine . . . . . . . . . . . . . . . . . . . 2 DL-Valine . . . . . . . . . . . . . . . . . . . 5 L-Lysine Hydrochloride .

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