By R. Ian Freshney(auth.)
This is the 6th version of the top textual content within the simple method of mobile tradition, all over the world. conscientiously revised, it positive factors updates on really expert thoughts in stem mobilephone examine and tissue engineering; updates on molecular hybridization, somatic mobile fusion, hybridomas, and DNA move; new sections on vitrification and Organotypic tradition, and new chapters on epithelial, mesenchymal, neurectodermal, and hematopoietic cells; germs cells/stemcells/amniocytes; and non-mammalian/avian cells. it's written for graduate scholars, learn and medical scientists, and technicians and laboratory managers in mobile and molecular biology labs and genetics labs.
PowerPoint slides of the figures in addition to different supplementary fabrics can be found at a significant other website: www.wiley.com/go/freshney/cellculture
Chapter 1 advent (pages 1–10):
Chapter 2 Biology of Cultured Cells (pages 11–23):
Chapter three Laboratory layout, format, and gear (pages 25–36):
Chapter four gear and fabrics (pages 37–56):
Chapter five Aseptic method (pages 57–70):
Chapter 6 defense, Bioethics, and Validation (pages 71–88):
Chapter 7 tradition Vessels and Substrates (pages 89–98):
Chapter eight outlined Media and supplementations (pages 99–114):
Chapter nine Serum?Free Media (pages 115–132):
Chapter 10 coaching and Sterilization (pages 133–162):
Chapter eleven fundamental tradition (pages 163–186):
Chapter 12 way of life and mobile strains (pages 187–206):
Chapter thirteen Cloning and choice (pages 207–225):
Chapter 14 cellphone Separation (pages 227–237):
Chapter 15 Characterization (pages 239–268):
Chapter sixteen Differentiation (pages 269–278):
Chapter 17 Transformation and Immortalization (pages 279–297):
Chapter 18 illness (pages 299–315):
Chapter 19 Cryopreservation (pages 317–334):
Chapter 20 Quantitation (pages 335–364):
Chapter 21 Cytotoxicity (pages 365–381):
Chapter 22 really good Cells (pages 383–432):
Chapter 23 Stem Cells, Germ Cells, and Amniocytes (pages 433–462):
Chapter 24 tradition of Tumor Cells (pages 463–479):
Chapter 25 Three?Dimensional tradition (pages 481–495):
Chapter 26 Scale?Up and Automation (pages 497–515):
Chapter 27 really good recommendations (pages 517–532):
Chapter 28 education courses (pages 533–568):
Chapter 29 challenge fixing (pages 569–591):
Chapter 30 In end (page 593):
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Additional info for Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Sixth Edition
A) Normal Human Keratinocytes on 3T3 Feeder Layer. Keratinocyte pancytokeratin stained green, and vimentin in 3T3 cells, red. ) (c) Breast Stromal Fibroblasts. Immunoperoxidase stained for vimentin (brown). ) (b) Human Glioma. MOG-G-CCM cells stained for GFAP by immunoperoxidase. (d) Human Umbilical Vein Endothelial cells. Factor VIII granular staining by immunoperoxidase. Plate 11. Immunostaining. (a) Dome Forming in Monolayer of Wil Lung Adenocarcinoma. Mosaic of CEA-positive and CEA-negative cells.
Harrison  chose the frog as his source of tissue, presumably because it was a cold-blooded animal, and consequently incubation was not required. Furthermore because tissue regeneration is more common in lower vertebrates, he perhaps felt that growth was more likely to 4 CULTURE OF ANIMAL CELLS 60000 Number of hits 50000 40000 30000 20000 10000 0 1960 Cumulative total [Fischer,1925] 1970 1980 1990 2000 2010 Pulication year Fig. 1. Growth of Tissue Culture. Number of hits in PubMed for ‘‘cell culture’’ from 1965.
A) (b) (c) (a–c) Magnetic Sorting with Dynabeads (Dynal). Negative sort; committed progenitor cells from bone marrow suspension bound to Dynal paramagnetic beads with antibodies to lineage markers. Lineage negative (stem) cells are not bound and remain in the suspension ready for sorting by flow cytometry. (a) Inserting the tube into the magnetic holder. (b) Tube immediately after being placed in magnetic holder. (c) Tube 30 s after placement in magnetic holder. (d) (e) (d, e) Magnetic Cell Sorting with the Midi-MACS Separator (Miltenyi Biotec).