Ciba Foundation Symposium 136 - Cell and Molecular Biology

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Brings jointly the newest examine within the mobile and molecular biology of bones and enamel, together with functions to clinical and dental perform. An interdisciplinary crew of participants addresses keep an eye on of bone formation, resorption and remodelling, osteoblast differentiation and osteoclast job, elements influencing dental extracellular matrix biomineralization, non-collagen proteins in bone and their functionality, hormonal legislation of bone progress, and extra.

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A) Adjacent colonies, colony on left completely negative for the enzyme, colony on right with large proportion of cells positive for the enzyme, and (b) colony with small proportion of cells positive for the enzyme. (c) Colony in culture treated with epidermal growth factor from Day 0. This colony is larger than in control culture and a negligible proportion of cells is positive for the enzyme. ( X 8) Reproduced by permission from Owen et a1 (1987). fewer cells positive for the enzyme arise from CFU-F which are earlier in the lineage.

2. Molecular features of 26 kDa amelogenin, showing: the predicted amino acid sequence based upon mouse and bovine amelogenin cDNA sequence data, (see Shimokawa et al 1987, Snead et a1 1985); the signal peptide cleavage point; cleavage of the conserved N-terminal 'TRAP' sequence at amino acid position 45/46 (A. Fincham, unpublished results); a proposed cleavage point at amino acid residues 62/63 (M. Crenshaw, personal communication); a cleavage point for the C-terminal telopeptide at residues 184/185 (M.

Stromal cell lines synthesize haemopoietic colony-stimulating factors and this has stimulated considerable interest in the characterization of these lines from marrow (Zipori et a1 1985, Lim et a1 1986, Rennick et a1 1987, Lanotte et a1 1982). Different culture conditions selectively promote the growth of different stromal cell populations. With suspensions of single marrow cells and fetal calf serum, the formation of fibroblastic colonies from precursors designated CFU-F has been confirmed for several species including man, in a number of laboratories.

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