Cellulases by Harry J. Gilbert (Eds.)

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By Harry J. Gilbert (Eds.)

Cellulase refers to a category of enzymes produced mainly by means of fungi, micro organism, and protozoans that catalyze cellulolysis. This quantity of Methods in Enzymology comprehensively covers this subject. With a global board of authors, this quantity covers topics such as ''The DNSA lowering assay for measuring cellulases,'' ''Measuring processivity'' and ''In situ cellulose detection with carbohydrate-binding modules.''

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    ABELSON VOLUME 318. RNA–Ligand Interactions (Part B: Molecular Biology Methods) Edited by DANIEL W. CELANDER AND JOHN N. ABELSON VOLUME 319. Singlet Oxygen, UV-A, and Ozone Edited by LESTER PACKER AND HELMUT SIES VOLUME 320. Cumulative Subject Index Volumes 290–319 VOLUME 321. Numerical Computer Methods (Part C) Edited by MICHAEL L. JOHNSON AND LUDWIG BRAND VOLUME 322. Apoptosis Edited by JOHN C. REED VOLUME 323. Energetics of Biological Macromolecules (Part C) Edited by MICHAEL L. JOHNSON AND GARY K.

    CM-cellulose 4M (Megazyme cat. no. P-CMC4M) cannot be used because it does not completely dissolve, and would thus block the capillary tube in the viscometer. While exo-acting enzymes can remove glucose from the reducing end of the polysaccharide, extensive hydrolysis is required before there is any effect on viscosity. Also, with chemically modified polysaccharides such as CM-cellulose 7M, hydrolysis by exo-acting enzymes is blocked by the carboxymethyl groups. Viscometric assays also offer high sensitivity; cleavage of a single bond per molecule can result in a dramatic decrease in viscosity.

    CELANDER AND JOHN N. ABELSON VOLUME 318. RNA–Ligand Interactions (Part B: Molecular Biology Methods) Edited by DANIEL W. CELANDER AND JOHN N. ABELSON VOLUME 319. Singlet Oxygen, UV-A, and Ozone Edited by LESTER PACKER AND HELMUT SIES VOLUME 320. Cumulative Subject Index Volumes 290–319 VOLUME 321. Numerical Computer Methods (Part C) Edited by MICHAEL L. JOHNSON AND LUDWIG BRAND VOLUME 322. Apoptosis Edited by JOHN C. REED VOLUME 323. Energetics of Biological Macromolecules (Part C) Edited by MICHAEL L.

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