Capillary Electrophoresis of Nucleic Acids Volume 2 by Mitchelson, Cheng

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By Mitchelson, Cheng

An exceptional panel of hands-on specialists and builders of CE gear describe in step by step style their top state of the art tools for the detection and research of DNA mutations and differences, starting from particular DNA loci to whole genomes of organisms. This moment quantity of the set, useful purposes of Capillary Electrophoresis, covers thoughts for high-throughput research of DNA fragments utilizing SNP detection, mutation detection, DNA sequencing equipment, and DNA-ligand interactions. numerous chapters additionally talk about CE research at increased temperatures and using micro-CE and array-CE units. The spouse quantity, advent to the Capillar Electrophoresis of Nucliec Acids, bargains with ease reproducible equipment for the research of small oligonucleotides and transformed nucleotides, and time-tested suggestion on instrumentation, sign detection, the capillary surroundings, and the combination of mass spectrometry with CE. entire and up to date, the paired volumes of Capillary Electrophoresis of Nucleic Acids provide an authoritative advisor with easy accessibility to speedy, flexible, trustworthy, and strong applied sciences for all these uncomplicated and scientific investigators reading DNA version this present day.

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Additional resources for Capillary Electrophoresis of Nucleic Acids Volume 2 Practical Applications of Capillary Electrophoresis (Methods in Molecular Biology Vol 163)

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Next, we examined the effect of raising the electrophoresis temperature from 30°C, in increments of 5, to 60°C (Table 1, #4–10). The effects on overall electrophoresis time are probably due to a decrease in polymer viscosity which reduces the capillary fill time and increases the speed by which DNA fragments migrate through the polymer mesh. 52 min. Peaks became increasingly broad at elevated run temperatures (above 50°C), which could have been caused by a partial denaturation of the dsDNA injected from water.

And Mao, D. T. (2001) Sieving matrix selection, in Capillary Electrophoresis of Nucleic Acids, Vol. 1 (Mitchelson, K. ), Humana Press, Totowa, NJ, pp. 167–187. 7. , and Foret, F. (1998) Ultrafast DNA analysis by capillary electrophoresis. Electrophoresis 19, 1436–1441. 8. , Williams, P. , Williams, S. , Brown, M. , Wallace, D. , and Karger, B. L. (1996) Fast capillary electrophoresis-laser induced fluorescence analysis of ligase chain reaction products: Human mitochondrial DNA point mutations causing Leber’s hereditary optic neuropathy.

5. Olivera, B. , and Davidson, N. (1964) Electrophoresis of nucleic acids. Biopolymers 2, 245–257. 6. , and Mao, D. T. (2001) Sieving matrix selection, in Capillary Electrophoresis of Nucleic Acids, Vol. 1 (Mitchelson, K. ), Humana Press, Totowa, NJ, pp. 167–187. 7. , and Foret, F. (1998) Ultrafast DNA analysis by capillary electrophoresis. Electrophoresis 19, 1436–1441. 8. , Williams, P. , Williams, S. , Brown, M. , Wallace, D. , and Karger, B. L. (1996) Fast capillary electrophoresis-laser induced fluorescence analysis of ligase chain reaction products: Human mitochondrial DNA point mutations causing Leber’s hereditary optic neuropathy.

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