By Mitchelson, Cheng
A good panel of hands-on specialists and builders of CE apparatus describe in step by step style their most sensible state-of-the-art tools for the detection and research of DNA mutations and changes, starting from specific DNA loci to whole genomes of organisms. this primary quantity of the set, creation to the Capillary Electrophoresis of Nucleic Acids, covers the sensible and theoretical issues at the back of using capillary electrophoresis for the research of small oligonucleotides and transformed nucleotides. besides particular directions making sure prepared reproducibility, those protocols provide time-tested suggestion on instrumentation, sign detection, the capillary atmosphere, and the mixing of mass spectrometry with CE. a number of chapters are dedicated to the research of small healing oligonucleotides, nucleosides, and ribonucleotides through CE. The spouse quantity, functional purposes of Capillary Electrophoresis, addresses options for high-throughput research of DNA fragments utilizing SNP detection, mutation detection, DNA sequencing tools, and DNA-ligand interactions. accomplished and updated, the paired volumes of Capillary Electrophoresis of Nucleic Acids supply an authoritative advisor with easy accessibility to speedy, flexible, trustworthy, and strong applied sciences for all these simple and medical investigators interpreting DNA edition this day.
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Extra info for Capillary Electrophoresis of Nucleic Acids Volume 1 Introduction to the Capillary Electrophoresis (Methods in Molecular Biology Vol 162)
3. , and Cheng, J. (2001) Microchip-based capillary electrophoresis systems, in Capillary Electrophoresis of Nucleic Acids, Vol. 1 (Mitchelson, K. R. ), Humana Press, Totowa, NJ, pp. 67–83. 4. McKenzie, S. , and Fortina, P. (1998) Parallel molecular genetic analysis. Eur. J. Hum. Genet. 6, 417–429. 5. Effenhauser, C. , Bruin, G. J, and Paulus, A. (1997) Integrated chip-based capillary electrophoresis. Electrophoresis 18, 2203–2213. 6. Eggers, M. and Ehrlich, D. (1995) A review of microfabricated devices for gene-based diagnostics.
Development of serial electrodes that provide for high “sweeping fields” separation using low-voltage supplies are suited to transportable and hand-held devices (121,122). Such pocket-sized devices have achieved PCR-amplification in 15 min and CE analysis in 2 min, to provide complete analysis in under 20 min (93,107), and will bring the analytical possibilities of CE in a easily transportable format. In the near future, real-time monitoring of PCR-amplification reactions with integrated microanalytical devices will allow direct diagnostic analysis such as quantification of gene dosage by PCR-RFLP analysis (41) and quantification of gene expression by QRT-PCR analysis to the doctors’ surgery and to the scientist in the field (93,120).
1997) Capillary electrophoretic mobility shift assay (CEMSA) a protein-DNA complex. J. Capillary Electrophor. 4, 225–231. 104. Foulds, G. J. and Etzkorn, F. A. (2001) Protein-DNA binding affinities by capillary electrophoresis, in Capillary Electrophoresis of Nucleic Acids, Vol. 2 (Mitchelson, K. R. ), Humana Press, Totowa, NJ, pp. 369–378. 105. Mangru, S. D. and Harrison, D. J. (1998) Chemiluminescence detection in integrated post-separation reactors for microchip-based capillary electrophoresis and affinity electrophoresis.