By Henry F. Epstein and Diane C. Shakes (Eds.)
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Extra info for Caenorhabditis elegans: Modern Biological Analysis of an Organism
1978) (NA22 is avail- 17 1. 31 Advantageddisadvantages High-quality nematodes with no special preparation but low yield High-quality nematodes prepared from readily available ingredients but modest yield High yield, inexpensive to prepare but worms may be difficult to purify from egg debris and tend to form dauers from overcrowding Large quantities prepared in minimal number of culture vessels, but requires special equipment and personnel training; worms grown in liquid appear somewhat starved compared with those grown on NGM plates a Cost calculations include only expendable materials, not capital equipement or labor.
And Epstein, H. F. (1978). Myosins exist as homodimers of heavy chains: Demonstration with specific antibody purified by nematode mutant myosin affinity chromatography. Cell 15, 405-411. Schauer, I. , and Wood, W. B. (1990). Early C. elegans embryos are transcriptionally active. Development 110,1303-1317. Stroeher, V. , Kennedy, B. , Millen, K. , Schroeder, D. , Hawkins, M. , and McGhee, J. D. (1994). DNA-protein interactions in the Caenorhabdiris elegans embryo: Oocyte and embryonic factors that bind to the promoter of the gut-specific ges-2 gene.
173, 599-607. Nelson, G. , Roberts, T. , and Ward, S. (1982). Caenorhabditis elegans spermatozoan locomotion: Amoeboid movement with almost no actin. J. Cell Biol. 92, 121-131. Riddle, D. L. (1988). The Dauer Larva. In “The Nematode Caenorhabditis elegans” (W. B. ), pp. 393-412. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York. Rogalski, T. , Bullerjahn, A. M. , and Riddle, D. L. (1988). Lethal and amanitin-resistance mutations in the Caenorhabditis elegans ama-1 and ama-2 genes. Genetics 120,409-422.