By Jean Brachet
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Extra info for Biology of Acetabularia
The sedimentation pattern of the RNA's, extracted from whole homogenates or from the chloroplastic pellet of adult, cap-bearing plants (in which the vegetative nucleus has disappeared), undergoes the same modifications as in the case of anucleate fragments. This observation supports the idea that the nucleus might control the normal level of both the 16 and 25 s RNA T s in the chloroplasts. Moreover, Farber et al. (1969) have shown that the ability of the Acetabularia RNA T s to stimulate protein synthesis in a cell-free system decreases during cap formation.
Brächet, Biochim. Biophys. Acta 6A_, 157 (1963). 4. B. R. Green, V. Heilporn, S. Limbosch, M. Boloukhere and J. Brächet, Proc. S. Nat. Acad. Sei. 58, 1351 (1967). 5. D. C. Shephard, Exp. Cell Res. 31_, 93 (1965). 6. V. Heilporn-Pohl and J. Brächet, Biochim. Biophys. Acta, 119, 429 (1966). 7. L. L. Vaughan and A. Rich, J. Mol. Bio!. 7_, 130 (1963). 8. R. Wells and M. Birnstiel, Biochem. J. 112, 777 (1969). 9. E. Baltus, J. Edstrom, M. Janowski, J. Hanocq-Quertier, R. Tencer and J. Brächet, Proc.
In a few spots, fi brils with a diameter of 35-40 A were found, suggesting the presence of single-stranded regions. g. stroma) disturbing the protein monolayer and probably contributing to a shadowing artifact. Plate 5 is part of another large display. The un coiled region is continuous with the coiled region. 3 for the latter to compensate for loss of length on twisting. This makes a total of 75 μ or 150 x 10" daltons for a small part which is only about one-tenth of a display. In Plate 6, another region of the same display, there is a ring formed by supercoiling.