By Cord C. Uphoff, Hans G. Drexler (auth.), Cheryl D. Helgason, Cindy L. Miller (eds.)
At a few element of their careers, almost each scientist and technician, in addition to many doctors, despite their zone of specialization have a necessity to make use of mobile tradition platforms. Updating and considerably increasing upon the former versions, Basic mobilephone tradition Protocols, Fourth Edition presents the beginner cellphone culturist with enough details to accomplish the elemental ideas, to make sure the well-being and id in their phone strains, and with a view to isolate and tradition really good basic mobile varieties. The motive of this vast quantity is to generate a necessary source containing transparent methodologies pertinent to present parts of research, instead of trying to teach mobilephone culturists on particular telephone kinds or organ platforms. Written within the hugely winning Methods in Molecular Biology™, chapters comprise introductions to their respective issues, lists of the required fabrics and reagents, step by step, easily reproducible laboratory protocols, and pointers on troubleshooting and warding off identified pitfalls.
Comprehensive and updated, Basic mobilephone tradition Protocols, Fourth Edition compiles the basic thoughts had to technique this very important laboratory job with complete success.
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Additional resources for Basic Cell Culture Protocols
Evaluation of the identities of over 500 cell lines from external customers shows that the incidence of intraspecies CC is ~10% while the incidence of interspecies CC is ~6%. These results highlight the need for a standard authentication procedure for cell line identity and purity testing. Researchers using transgenic animal technology utilize various rodent cell lines. The most relevant cell lines for the biotechnology industry, BHK-21 and CHO, were derived from Syrian and Chinese hamsters, respectively.
Thus, the cell status should be frequently examined under the inverted microscope. 2. After passaging, test the cultures for mycoplasma contamination. If the cells are clean, freeze and store aliquots in liquid nitrogen. The cells in active culture have to be retested periodically to ensure continued freedom from mycoplasma contamination (see Note 7). C. G. Drexler 3. After complete decontamination, expand the cells and freeze master stocks of the mycoplasma-free cell line and store them in liquid nitrogen to provide a continuous supply of clean cells.
Chapter 2 Eradication of Mycoplasma Contaminations Cord C. Uphoff and Hans G. Drexler Abstract Mycoplasma contaminations have a multitude of effects on the cultured cell lines that may influence the results of experiments or pollute bioactive substances used in human medicine. The elimination of mycoplasma contaminations of cell cultures has become a practical alternative to discarding and reestablishing important or irreplaceable cell lines. Different quinolones, tetracyclines, and pleuromutilins shown to have strong antimycoplasma properties are employed for the decontamination.