Applied Genetics of Leguminosae Biotechnology by Randy D. Dinkins, M. S. Srinivasa Reddy, Curtis A. Meurer,

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By Randy D. Dinkins, M. S. Srinivasa Reddy, Curtis A. Meurer, Carl T. Redmond (auth.), Pawan K. Jaiwal, Rana P. Singh (eds.)

Legumes comprise many vitally important crop crops that give a contribution very serious protein to the diets of either people and animals world wide. Their designated skill to mend atmospheric nitrogen in organization with Rhizobia enriches soil fertility, and establishes the significance in their area of interest in agriculture. Divided into volumes, this paintings provides an up to date research of in vitro and recombinant DNA applied sciences for the development of grain, forage and tree legumes.

Volume 10B provides the present kingdom and destiny customers of in vitro regeneration and genetic transformation expression and balance of transgenes amendment of qualities in just about all the $64000 legumes, for instance: soybean; peanut; pea; french bean; chick pea; pigeon pea; cowpea; mung bean; black gram; azuki bean; lentil; Lathyrus; lupinus; Lotus spp; Medicago spp; Trifolium spp; Winged bean; Guar; and tree legumes for his or her development.

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80: 246 -252. Puonti-Kaerlas J, Eriksson T and Engstrom P (1992) Inheritan ce of a bacte rial hygromycin pho sphotransferase gene in the progeny of primary transgenic pea plant s. App l. Genet. , 84: 443 -450. Pusztai A, Bardoc z G G S, Alonso R, Chrispeels M J, Schro ede r H E, Tabe L M and Higgin s T J V (1999) Express ion of the insecticidal bean alpha-am ylase inhibitor transgene has minimal detrimental effect on the nutrition al value of peas fed to rats at 30% of the diet. Journal ofNutrition , 129: 1597-1603 .

1999) also used a modified Schroeder et al. (1993) method which uses embryonic axes from mature seed. They used the Agrobacterium strain AGL 1 and reported that the critical factors for them were the plant genotype, the explant, drying the explant after co-cultivation and using thiadiazuron (TDZ) in the regeneration med ium. They reported one experiment where they verified eight transformed shoots from fifty seeds. However, these were not confirmed by Southern analysis and experience in our laboratory has shown that there can be wide experiment to experiment variation, so these results would need to be repeated.

Jaiwal and R. ) , Applied Genetics ofLeguminosa e Biotechnology, 23-34. © 2003 KluwerAcademic Publishers. Jan E. Grant and Pauline A. , 1991). Incremental progress was made. , 1992). Since then a number of reports have been published for different and improved methods of pea transformation. Many factors are important in successful transformation and many facets of the process are not fully understood, so plant transformation , especially in legumes, remains more an art than an exact science (Birch, 1997; Gelvin, 2000) .

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