Analysis of Nucleic Acids by Capillary Electrophoresis by Christoph Heller (auth.), Christoph Heller (eds.)

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By Christoph Heller (auth.), Christoph Heller (eds.)

During the decade, capillary electrophoresis has been constructed right into a very robust analytical strategy, which has many merits over traditional slab gel strategies. the advance is similar to the only occuring prior within the box of chromatography, with the creation of the excessive functionality know-how. How very important this system has be­ come, is mirrored via the shear quantity of papers released every month; in addition to a dozen of books already released in this topic. probably the most very important meetings within the box, the "International Symposium on excessive functionality Capillary Electrophoresis" draws now approximately thousand humans each year. As capillary electrophoresis should be utilized to many various analytical difficulties, a spe­ cialization is unavoidable. This evolution can also be mirrored within the improvement of instrumen­ tation: while the 1st units have been designed for all attainable purposes, new tools at the moment are equipped, which are really expert for one specific activity, e.g. DNA research. I greatly welcome the choice of the sequence editor and the writer, to edit a sequence ofspecialized books, masking all points of capillary electrophoresis. Having labored at the electrophoretic separation of DNA for a few years, i'm confident that there are such a lot of diversified elements in this factor that they deserve a complete e-book all alone. hence, i used to be chuffed to agree whilst being requested to edit this book.

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20)-(21). Th e seco nd term in Eq. (24) is the sm all contribution of the field to the orientation of the random-walk tube . The limiting mobilit y and the loss of separation in Eq . (25) are due to the fact that when the tube orientation is important, which is the case for large DNA rnoleeules No > N* , one gets h, - L, and Eq . (23) then pred icts a size- independent mobility. Avoiding tube orientation is thus the main goal of pulsed field techniques. Since Eq . (24) is a decreasing function of No while Eq .

A. Me Gregor and E. S. Yeung, "Optimization of capillary electrophoretic separation of DNA fragments based on polymer filled capillaries", J. Chromatography A, 6521993 67-73. [74] S. Nathakarnkitkool, P. 1. Oefner, G. Bartsch, M. A. Chin and G. K. Bonn , "High -resolution capill ary electrophoretic analysis of DNA in free solution", Electrophoresis, 13 1992 18-31. [75] M. H. KleemiB, M. Gilge s and G. Schomburg, "Capillary electrophoresis of DNA restriction fragments with solutions of entangled polymers", Electrophoresis, 14 1993 515 -522 .

24) is a decreasing function of No while Eq . (25) is an increasing one , the mobility is predi cted to attain a minimum value for an intermediate molecular size [9, 10, 77] 14 Nmin "? (27) This was a very surprising pred icti on of the BRM . Indeed, the minimum, which is also called the "band inversion" phenomenon since it impl ies that the electrophoreti c band s may not be found in the "right" ord er on the gel, was unknown to all pract itioners! Thi s minimum was found experimentally in agarose gel electroph oresis [9, 10], and later reported in pol yacrylamid e gels for ssDNA [70,78].

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